ZEARALENONE Elisa Kit

ZEARALENONE Elisa Kit has lower price but nearly the same quality compared to that made by Europea or USA.

1.         INTENTED USE

Quicking Zearalenone ELISA Kit is a direct method competitive enzyme-linked immunoassay for the quantitative detect the presence of Zearalenone in samples of grains and feed.  

2.         PRINCIPLE OF THE ASSAY

Quicking Zearalenone ELISA kit is a direct method competitive enzyme-linked immunoassay. The assay is performed in ELISA microwells, which have been precoated with one anti-Zearalenone antibodies. When standards or samples are added into the microwells, the free Zearalenone antigens and the enzyme conjugate will compete to combine the antibody binding site on the microwells during incubation. After that, the unbound materials will be removed in a washing step. Then, two substrates are added into the microwells. The bound enzyme conjugate will convert the colorless substrate into blue. The color will change from blue to yellow after adding stop solution. Absorbance is then measured spectrophotometrically (450 nm) and colour intensity results are inversely proportional to the original Zearalenone amount in the sample. Sample concentrations are then calculated on the basis of a calibration curve derived from the standards.

3.         KIT COMPONENT

-           1 X Microtiter plate (8 microwells X 12 removable strips) precoated with anti-Zearalenone antibodies

-           6 X Zearalenone standard solutions (1 mL each): 0 ng/mL,2 ng/mL, 4 ng/mL, 8 ng/mL, 20 ng/mL, 100 ng/mL

-           1 X Enzyme-linked conjugate  (6 mL)

-           1 X Washing buffer concentrate (10 X , 50 mL)

-           1 X Substrate A: Carbamide Peroxide, (6 mL)

-           1 X Substrate B: TMB solution (6 mL)

-           1 X Stopping solution, 2 M Sulphuric acid (6 mL)

-           Product Manual

4.         PREPARATION OF WORKING SOLUTIONS

5.         SAMPLE PREPARATION

6.         ELISA TESTING PROTOCOL

7.         CALCULATION

A special program with Excel functionality, Quicking ELISA Analysis Program in Excel, is available for giving the ELISA test results.

8.         STORAGE

-           The kit should be stored at 2~8°C, NO FREEZING.

-           Unused test wells should be sealed and dryly stored.

9.         LIMITATION

Quicking ZEARALENONE Elisa Kit ia used as a screening system. If a more accurate result is required, other methods such as HPLC or GC/MS is suggested to give a confirmation.

ZEARALENONE Elisa Kit