Penicillin Rapid Test (Milk)

Quicking Penicillin Rapid Test has lower price but nearly the same quality compared to that made by European or USA.

Penicillin G Rapid Test (Milk)

Cat No.: W81046-1

1.        INTENDED USE

Quicking Penicillin G Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Penicillin G residue in milk.

Cut-off:   4 ppb

Assay Time:  5 – 10 min

Sample: Defatted milk

2.        PRINCIPLE OF THE ASSAY

Quicking Penicillin G Rapid Test is based on competitive lateral flow immunochromatographic assay. The Penicillin G conjugate in the test zone will capture the immuno-gold (colloid gold-Penicillin G antibody conjugate), when there is very little dissociative Penicillin G in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Penicillin G is present in concentration of 4 ppb and above which explains a positive result.

3.        KIT COMPONENT

-           10×foil pouches each contain one cassette and a desiccant

-           20×pipettes

-           10×centrifugal tubes (1.5 mL)

-           1×plastic canister containing 10 microwells and a desiccant

-           Product Manual

4.        TEST PROCEDURE

-           Bring 2-3 mL of the under tested milk to room temperature (20-30°C).

-           Collect 1mL of milk sample into a centrifugal tube. Do centrifugation at 7000 rpm for 3-4 min to separate the fat component.

-           Take out the microwells strip from the plastic canister. Take one well and tear off the film.

-           Insert the pipette through the fat layer (5 mm below) to collect the defatted milk towards the reticle in the pipette (200 μL) and transfer it into the well.

-           Use another pipette to repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for about 2 min. NOTE: The sensitivity of the strip is positive correlated to the reaction time. It is suggested to wait for 1-2 min before running the assay.

-           Take out the cassette from the foil pouch and place it horizontally.

-           Suck the mixture in the well and gradually drip 3 drops into the assay sample hole “S”.

-           Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

5.        INTERPRETATION OF RESULTS

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Penicillin G is at or above 4 ppb in the samples.

Negative: The presence of both clear band in C zone and T zone.

Invalid: No colored band appears in C zone.

6.        SPECIFICITY

The results are negative when the test card is applied to detect 100 ppm of Chloramphenicol, Aminoglycosides, Tetracyclines, Macrolides, Sulfonamides, Cephapirin and Ceftiofur; 200 ppb of  Amoxicillin; 50 ppb of Cloxacillin and 20 ppb of Ampicillin.

7.        STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8.        PRECAUTIONS

-           For best results, please strictly adhere to these instructions.

-           All reagents must be at room temperature before running the assay.

-           Do not remove test cassette from its pouch until immediately before use.

-           Do not reuse the test kit.

-           Do not use the test beyond its expiration date marked on the foil pouch.

-           The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

9.        LIMITATION

Quicking Penicillin G Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Penicillin G above 4 ppb in milk. If you want a quantitative result, it is suggested to apply other method such as ELISA / HPLC in practice.

Penicillin Rapid Test (Milk)