Ractopamine Residue Rapid Test (Meat, 5ppb)

1.Fast and accurate 2.Read in 5min 3 OEM:available 4.Very Easy to Use 5.Certification:China Agriculture Dept &ISO 9001

Ractopamine Rapid Test (Tissue)

Cat No.: W81041-2

1.        INTENDED USE

Quicking Ractopamine Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Ractopamine residue in animal’s tissue.

Cut-off:   5 ppb

Assay Time:  5 -10 min

Sample: Tissue

2.        PRINCIPLE OF THE ASSAY

Quicking Ractopamine Rapid Test is based on competitive lateral flow immunochromatographic assay. The RAC-conjugate in the test zone will capture the immuno-gold (colloid gold-RAC antibody conjugate), when there is very little dissociative RAC in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if RAC is present in concentration of 5 ppb and above which explains a positive result.

3.        KIT COMPONENT

-           10×foil pouches each containing one cassette with one pipette and a desiccant

-           1×assay diluent (8 mL) 

-           12×centrifugal tubes (2×,15 mL; 10×,1.5 mL)

-           2×graduated pipettes (3 mL )

-           Products Manual

4.        TEST PROCEDURE

-           Collect tissue as sample. Get rid of the fat tissue. Homogenize the sample at 10000 rpm for 1 min. Weigh out 4.0 g of sample into a 15 mL centrifugal tube. Add 0.5 mL of assay diluent and tightly cover the lid.

-           Put the tube into a water bath (80°C ) for 10 min. Take out as much the extracted liquid into a 1.5 mL centrifugal tube.

-           Do centrifugation at 4000 rpm for 1 min to make the extract clear. NOTE: If there is fat layer, just insert the pipette through the fat layer and take the water phase liquid for running the assay.

-           Take out the cassette from the foil pouch and place it horizontally.

-           Gradually drip 4 drops of sample extraction into the sample hole “S”.

-           Interpret the result after 5 min. Result after 10 min is considered as invalid.

5.        INTERPRETATION OF RESULTS

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Ractopamine is at or above 5 ppb in the sample.

Negative: The presence of both clear band in C zone and T zone.

Invalid: No colored band appears in C zone.

6.        SPECIFICITY

The results are negative when the test is applied to detecting 100 ppm of Clenbuterol, Salbutamol, Trenbolone-acetate, Chloramphenicol, Aminoglycosides, Beta-Lactams, Macrolides, Quinolones, and Tetracyclines.

7.        STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8.        PRECAUTIONS

-           For best results, please strictly adhere to these instructions.

-           All reagents must be at room temperature before running the assay.

-           Do not remove test cassette from its pouch until immediately before use.

-           Do not reuse the test kit.

-           Do not use the test beyond its expiration date marked on the foil pouch.

-           The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

9.        LIMITATION

Quicking Ractopamine Rapid Test is a useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Ractopamine above 5 ppb in meat. If you want a quantitative result, please adopt other method such as ELISA  in practice.

Ractopamine Residue Rapid Test (Meat, 5ppb)