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- Company Name : Quicking Biotech Co., Ltd.
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Quicking Clenbuterol residue rapid test kit(tissue,3ppb) (ISO9001+ISO13485)
1. INTENDED USE
Quicking Clenbuterol Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Clenbuterol residue in animal’s tissue.
Cut-off: 1,3 ,5ppb
Assay Time: 5 -10 min
Sample: Tissue
2. PRINCIPLE OF THE ASSAY
Quicking Clenbuterol Rapid Test is based on competitive lateral flow immunochromatographic assay.
3. TEST PROCEDURE
3.1Collect tissue as sample. Get rid of the fat tissue. Homogenize the sample at 10000 rpm for 1 min. Weigh out 4.0 g of sample into a 15 mL centrifugal tube. Add 0.5 mL of assay diluent and tightly cover the lid.
3.2 Put the tube into a water bath (80°C ) for 10 min. Take out as much the extracted liquid into a 1.5 mL centrifugal tube.
3.3 Do centrifugation at 4000 rpm for 1 min to make the extract clear. NOTE: If there is fat layer, just insert the pipette through the fat layer and take the water phase liquid for running the assay.
3.4 Take out the cassette from the foil pouch and place it horizontally.
3.5 Gradually drip 3 drops of sample extraction into the sample hole “S”.
3.6 Interpret the result in 5 - 10 min. Result after 10 min is considered as invalid.
4. INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Clenbuterol is at or above 1,3,5 ppb in the sample.
Negative: The presence of both clear band in C zone and T zone.
Invalid: No colored band appears in C zone.
5. SPECIFICITY
- The results are negative when the test is applied to detect 100 ppm of Ractopamine, Trenbolone-acetate, Chloramphenicol, Aminoglycosides, Beta-Lactams, Macrolides, Quinolones, and Sulfonamides.
- The results is negative when the test is applied to detect 1 ppm of Salbutamol. .
6. STORAGE
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.
Quicking Clenbuterol residue rapid test kit(tissue,3ppb) (ISO9001+ISO13485)